Virus:Article Title: Microbial Siderophore Enterobactin Promotes Mitochondrial Iron Uptake and Development of the Host via Interaction with ATP Synthase.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATP5A1 Monoclonal Antibody (15H4C4) Thermo Fisher Cat #43-9800 RRID:AB_2533548 ATP5B Polyclonal Antibody (A5769) Abclonal Cat #A5769 Rabbit polyclonal anti-actin antibody Sigma Cat #A2066 RRID:AB_476693 Streptavidin-HRP Cell Signaling Tech Cat #3999 RRID: AB_10830897 Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP Thermofisher Cat #62-6520 RRID:AB_88369 Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) Jackson ImmunoResearch Laboratories Cat #111-035-003 RRID:AB_2313567 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Invitrogen Cat #A-11011 RRID:AB_143157 Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat #A-11001 RRID:AB_2534069 Bacterial and Virus Strains E. coli Keio Knockout Parent Strain BW25113 Dharmacon Cat #OEC5042 E. coli Keio Knockout Collection Dharmacon Cat #OEC4988 Escherichia coli OP50 Caenorhabditis Genetics Center (CGC) RRID:WB-STRAIN:OP50 RNAi feeding bacterial strain HT115(DE3) GE Dharmacon (ORF RNAi library) N/A RNAi feeding bacterial strain HT115(DE3) Source BioScience (Ahringer) N/A Chemicals, Peptides, and Recombinant Proteins Biotin congated enterobactin Elizabeth M. Nolan lab Enterobactin Sigma Cat #E3910-1MG Pyoverdines Sigma Cat #P8124-1MG Ferrichrome Iron-free from Ustilago sphaerogena Sigma Cat #F8014-1MG 2,3-Dihydroxybenzoic acid Sigma Cat #126209-5G Hemin Sigma Cat #51280-1G Ethylenediaminetetraacetic acid calcium disodium salt Sigma Cat #ED2SC Iron(III) chloride hexahydrate Sigma Cat #236489 Iron-55 Radionuclide, Ferric Chloride in 0.5M HCl PerkinElmer Cat #NEZ043002MC Calcein, AM, cell-permeant dye Thermo Fisher Cat #C3100MP Rhodamine B-[(1,10-phenanthrolin-5-yl)aminocarbonyl]benzyl ester Axxora Cat #SQX-RPA.1 MitoTracker Red CMXRos Cell Signaling Tech Cat #9082S Dynabeads M-280 Streptavidin Thermo Fisher Cat #11205D Human ATP5A1 recombinant protein (N-terminal 6xHis-tagged) MyBioSource Cat #MBS957551 C.elegans ATP-1 recombinant protein (N-terminal 6xHis-tagged) This paper N/A Peptide-1: IGRGQRELIIGDRQTGKTAI Genscript N/A Peptide-2: AIDTIINQKRFNDAGDDKKKL Genscript N/A Peptide-3: FCIYVAVGQKRSTVAQIVKRL Genscript N/A Peptide-4: LQFLAPYSGCAMGEHFRDNGK Genscript N/A (Continued on next page) Cell 175, 1–12.e1–e5, October 4, 2018 e1
Knock-Out:Article Title: Microbial Siderophore Enterobactin Promotes Mitochondrial Iron Uptake and Development of the Host via Interaction with ATP Synthase.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATP5A1 Monoclonal Antibody (15H4C4) Thermo Fisher Cat #43-9800 RRID:AB_2533548 ATP5B Polyclonal Antibody (A5769) Abclonal Cat #A5769 Rabbit polyclonal anti-actin antibody Sigma Cat #A2066 RRID:AB_476693 Streptavidin-HRP Cell Signaling Tech Cat #3999 RRID: AB_10830897 Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP Thermofisher Cat #62-6520 RRID:AB_88369 Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) Jackson ImmunoResearch Laboratories Cat #111-035-003 RRID:AB_2313567 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Invitrogen Cat #A-11011 RRID:AB_143157 Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat #A-11001 RRID:AB_2534069 Bacterial and Virus Strains E. coli Keio Knockout Parent Strain BW25113 Dharmacon Cat #OEC5042 E. coli Keio Knockout Collection Dharmacon Cat #OEC4988 Escherichia coli OP50 Caenorhabditis Genetics Center (CGC) RRID:WB-STRAIN:OP50 RNAi feeding bacterial strain HT115(DE3) GE Dharmacon (ORF RNAi library) N/A RNAi feeding bacterial strain HT115(DE3) Source BioScience (Ahringer) N/A Chemicals, Peptides, and Recombinant Proteins Biotin congated enterobactin Elizabeth M. Nolan lab Enterobactin Sigma Cat #E3910-1MG Pyoverdines Sigma Cat #P8124-1MG Ferrichrome Iron-free from Ustilago sphaerogena Sigma Cat #F8014-1MG 2,3-Dihydroxybenzoic acid Sigma Cat #126209-5G Hemin Sigma Cat #51280-1G Ethylenediaminetetraacetic acid calcium disodium salt Sigma Cat #ED2SC Iron(III) chloride hexahydrate Sigma Cat #236489 Iron-55 Radionuclide, Ferric Chloride in 0.5M HCl PerkinElmer Cat #NEZ043002MC Calcein, AM, cell-permeant dye Thermo Fisher Cat #C3100MP Rhodamine B-[(1,10-phenanthrolin-5-yl)aminocarbonyl]benzyl ester Axxora Cat #SQX-RPA.1 MitoTracker Red CMXRos Cell Signaling Tech Cat #9082S Dynabeads M-280 Streptavidin Thermo Fisher Cat #11205D Human ATP5A1 recombinant protein (N-terminal 6xHis-tagged) MyBioSource Cat #MBS957551 C.elegans ATP-1 recombinant protein (N-terminal 6xHis-tagged) This paper N/A Peptide-1: IGRGQRELIIGDRQTGKTAI Genscript N/A Peptide-2: AIDTIINQKRFNDAGDDKKKL Genscript N/A Peptide-3: FCIYVAVGQKRSTVAQIVKRL Genscript N/A Peptide-4: LQFLAPYSGCAMGEHFRDNGK Genscript N/A (Continued on next page) Cell 175, 1–12.e1–e5, October 4, 2018 e1
Recombinant:Article Title: Microbial Siderophore Enterobactin Promotes Mitochondrial Iron Uptake and Development of the Host via Interaction with ATP Synthase.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATP5A1 Monoclonal Antibody (15H4C4) Thermo Fisher Cat #43-9800 RRID:AB_2533548 ATP5B Polyclonal Antibody (A5769) Abclonal Cat #A5769 Rabbit polyclonal anti-actin antibody Sigma Cat #A2066 RRID:AB_476693 Streptavidin-HRP Cell Signaling Tech Cat #3999 RRID: AB_10830897 Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP Thermofisher Cat #62-6520 RRID:AB_88369 Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) Jackson ImmunoResearch Laboratories Cat #111-035-003 RRID:AB_2313567 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Invitrogen Cat #A-11011 RRID:AB_143157 Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat #A-11001 RRID:AB_2534069 Bacterial and Virus Strains E. coli Keio Knockout Parent Strain BW25113 Dharmacon Cat #OEC5042 E. coli Keio Knockout Collection Dharmacon Cat #OEC4988 Escherichia coli OP50 Caenorhabditis Genetics Center (CGC) RRID:WB-STRAIN:OP50 RNAi feeding bacterial strain HT115(DE3) GE Dharmacon (ORF RNAi library) N/A RNAi feeding bacterial strain HT115(DE3) Source BioScience (Ahringer) N/A Chemicals, Peptides, and Recombinant Proteins Biotin congated enterobactin Elizabeth M. Nolan lab Enterobactin Sigma Cat #E3910-1MG Pyoverdines Sigma Cat #P8124-1MG Ferrichrome Iron-free from Ustilago sphaerogena Sigma Cat #F8014-1MG 2,3-Dihydroxybenzoic acid Sigma Cat #126209-5G Hemin Sigma Cat #51280-1G Ethylenediaminetetraacetic acid calcium disodium salt Sigma Cat #ED2SC Iron(III) chloride hexahydrate Sigma Cat #236489 Iron-55 Radionuclide, Ferric Chloride in 0.5M HCl PerkinElmer Cat #NEZ043002MC Calcein, AM, cell-permeant dye Thermo Fisher Cat #C3100MP Rhodamine B-[(1,10-phenanthrolin-5-yl)aminocarbonyl]benzyl ester Axxora Cat #SQX-RPA.1 MitoTracker Red CMXRos Cell Signaling Tech Cat #9082S Dynabeads M-280 Streptavidin Thermo Fisher Cat #11205D Human ATP5A1 recombinant protein (N-terminal 6xHis-tagged) MyBioSource Cat #MBS957551 C.elegans ATP-1 recombinant protein (N-terminal 6xHis-tagged) This paper N/A Peptide-1: IGRGQRELIIGDRQTGKTAI Genscript N/A Peptide-2: AIDTIINQKRFNDAGDDKKKL Genscript N/A Peptide-3: FCIYVAVGQKRSTVAQIVKRL Genscript N/A Peptide-4: LQFLAPYSGCAMGEHFRDNGK Genscript N/A (Continued on next page) Cell 175, 1–12.e1–e5, October 4, 2018 e1
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